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. 2021 Mar 8;21:157. doi: 10.1186/s12935-021-01817-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Sphere culture caused enrichment of CD133 ⁺ population. The morphology of parental SK-UT-1 cells and spheres (magnification, ×200) (a), sphere formation rate of primary sphere and spheres at passages 1–4 on the 6 day (b), the process of formation of spheres derived from the SK-UT-1 cells (magnification, ×400) (c), SK-UT-1 derived-spheres enriched CD133 cells (d). The primary spheres and spheres at passages 1–4 were stained with FITC-conjugated antibody against CD133 and analyzed using a flow cytometer. Data were presented as mean ± SD (n = 3, *P < 0.05)