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. 2021 Mar 8;18:11. doi: 10.1186/s12979-021-00223-2

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Monocyte inflammatory gene expression profiles in Kenyan adults and children. Targeted digital RNA sequencing was performed on monocytes isolated from cryopreserved PBMCs via negative selection. A customized panel targeted 508 genes important in inflammation and immunity (QIAseq Human Inflammation and Immunity Transcriptome Panel, Qiagen). a Principal components analysis (PCA) plot of targeted digital RNA sequencing results for Kenyan adult (red) and child (blue) monocytes. b Heatmap of the top 50 most significantly differentially expressed genes between Kenyan adult and child monocytes