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. 2021 Mar 7;21:241. doi: 10.1186/s12885-021-07979-2

Fig. 2.

Fig. 2

a. Percentage of ALDHhigh cells in parental and hybrid cell lines evaluated by flow cytometry. * p value < 0,05; n = 4. b. Number of spheres after 10 days of culture in non-adherent condition. n = 6 c. Evaluation of stem cell frequency by ELDA method in spheres formed to evaluate self-renew capacity. d. Expression of NANOG and OCT4 by western blotting analysis in the monolayer culture of each cell lines. β-actin was used as loading control. Full-length blots are presented in Supplementary Fig. 4