FIGURE 4.

Deletion of MoBre2, MoSpp1, and MoSwd2 results in defects in hyphal growth, and pathogenic infection. (a) Five‐day‐old cultures of the wild type (WT) P131, Δmobre2, Δmospp1, and Δmoswd2 strains grown on oatmeal tomato agar (OTA). (b) Barley leaves sprayed with conidial suspensions (10⁵ spores/ml) of P131 and the Δmobre2, Δmospp1, and Δmoswd2 deletion mutants 36 hr after inoculation. (c–e) Microscopic observation of conidium formation in the wild‐type strain P131 and the Δmobre2, Δmospp1, and Δmoswd2 deletion mutants. IH, infection hypha; AP, appresorium. Means and SDs were calculated from three independent experiments. *p < .01, n > 100