FIGURE 2.

Coat protein (CP) of potato virus X (PVX) is detected in the apoplast isolated from PVX‐green fluorescent protein (PVX‐GFP)‐infected leaves. Western blot showing that PVX CP is specifically detected in the apoplast isolated from PVX‐GFP‐infected Nicotiana benthamiana. GFP, P25 (triple gene block protein 1), and actin were detected in the total proteins of PVX‐GFP‐infected leaves, but not in the apoplast, showing that there was no cytoplasmic contamination of the apoplast sample. Equal amounts of proteins were loaded in each case and Coomassie brilliant blue (CBB) staining of an equal sample was used as loading control. (Overexposed images are shown in Figure S2)