FIGURE 3.

Viral RNA and viral particles are present in the apoplast isolated from potato virus X (PVX)‐infected Nicotiana benthamiana leaves. (a) Agarose gel electrophoresis showing the reverse transcription PCR product of the coat protein (CP) region. (b) Northern blot using CP probe showing that PVX genomic RNA was present in the apoplast isolated from PVX‐green fluorescent protein (PVX‐GFP)‐infected N. benthamiana leaves, with total RNA isolated from PVX‐GFP‐infected leaves as control. (c) Transmission electron microscopy showing that PVX particles of the expected size were observed in the apoplast. The red arrow points to PVX viral particles. Scale bar represents 500 nm