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. 2021 Feb 24;22(4):456–464. doi: 10.1111/mpp.13039

FIGURE 4.

FIGURE 4

Potato virus X (PVX) coat protein (CP) is not released to the foliar apoplast by transient expression of CP:green fluorescent protein (GFP) or unfused CP. (a) CP:GFP colocalized with the plasma membrane with or without plasmolysis (FM4‐64 staining, red fluorescence). White arrowhead indicates the space after plasmolysis. Bar represents 50 μm. (b) In Nicotiana benthamiana leaves with transient expression of CP:GFP, western blot showed that CP:GFP could not be detected in the apoplast but only in the total protein isolated from the leaves. Coomassie brilliant blue (CBB) staining of equal volume was used in sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) as loading control (Figure S4a). (c) Western blot result showing the transient expression of CP in both the total protein and apoplastic fluid fractions. CBB staining of equal volume loaded SDS‐PAGE was used as loading control (Figure S4b)