A–C: The mRNA expressions of IL1A, IL6 and TNF-α in mouse prostatic tissues. Mice were fed with high fat diet (HFD) or regular fat diet (RFD), respectively for 4 months. The prostatic tissues were collected, and mRNA was extracted for qPCR. D–F: The mRNA expressions of IL1A, IL6 and TNF-α in human prostatic tissues from overweight patients (BMI≥25) and normal weight control (BMI<25). G–I: The mRNA expressions of IL1A, IL6 and TNF-α in macrophages. Macrophages were activated, followed by administration of myristic acid (MA) at the concentration of 0.25 μM, 1 μM and 2.5 μM for 12 hours. The data represent means of average determinants ± SEM. All experiments were repeated independently at least three times, with similar results. ***P < 0.001, ****P< 0.0001, compared with RFD group in mice tissues), compared with BMI<25 group in human tissues, and compared with vehicle in stroma cells.