Figure 5.

Human carboxylesterase 2 (CES2) improves triglyceride (TG) and glucose homeostasis in db/db mice. Twelve-week-old male db/db mice were intravenously injected with AAV8-ALB-Null or AAV8-ALB-CES2 and fed a chow diet for 12 wk (n = 7–8 mice per group). Hepatic triacylglycerol hydrolase (TGH) activity (A) and newly synthesized TG (B) were determined. Hepatic levels of mRNA (C) and proteins (D and E) related to lipid metabolism were analyzed. Postprandial and fasting glucose levels were measured (F). GTT (G) and pyruvate-tolerance test (PTT) (H) were performed. Hepatic levels of mRNA (I) and proteins (J and K) related to glucose metabolism were determined. *P < 0.05, **P < 0.01. AAV, adeno-associated virus.