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. 2021 Mar 8;778:146270. doi: 10.1016/j.scitotenv.2021.146270

Fig. 3.

Fig. 3

Bio-accumulation of heat-inactivated SARS-CoV-2 in oysters. Three batches of C. gigas oysters (B1112, B1113, B1114) were incubated for 24 h in presence of TuV, PEDV and heat-inactivated (in.) SARS-CoV-2. A. The viral concentration was quantified in three tissues - mantle (MT), digestive tissues (DT) and gills (GL) - by duplicate extractions using the PK-ISO method. ****: p < 0.0001, ns: non-significant (ANOVA), n = 2 series of extractions. In B1112 and B1113, PEDV or SARS-CoV-2 were not detected (ND) in some tissues. Theoretical limit of detection: 50 gc/g (dotted line). B. The virus concentration of in each tissue was divided by the initial virus concentration in the seawater to calculate the bio-accumulation index. Each oyster batch is plotted as a black symbol (circle, B1112; triangle, B1113; square, B1114) when the virus was detected in the corresponding tissue, missing symbols corresponding to undetected virus. The arithmetic mean values of the three experiments are plotted as columns, for the three tissues. ****: p < 0.0001, ns: non-significant (ANOVA), n = 3 experiments with different oyster batches.