Table C.1.
Histological evaluation of potential target tissues of HBCDDs toxicity in mice after 28 days of repeated dietary exposure to HBCDDs at daily doses of 49.5 μg/kg bw per day or 199 mg/kg bw per day (Maranghi et al., 2013; Rasinger et al., 2014, 2018). Significant effects at the p < 0.05 and p < 0.01 levels are indicated by (+) and (++), respectively
| Tissue | Observations | Control | HBCDDs low dose (49.5 μg/kg bw per day) | HBCDDs high dose (199 mg/kg bw per day) |
|---|---|---|---|---|
| Thyroid | Desquamation into follicular lumen | 0/7 (0%) | 3/7 (43%) | 1/7 |
| Foaming colloid | 1/7 (14%) | 2/7 (29%) | NA | |
| Uterus | Irregular multistratification of the luminal epithelium | 1/8 (12%) | 2/7 (29%) | NA |
| Reduction in endometrial glands density | 0/8 (0%) | 4/7(+) (57%) | NA | |
| Liver | Marked vacuolisation in hepatocytes | 0/10 (0%) | 5/8(++) (62%) | 5/8(++) |
| Lymphocytic infiltration | 0/10 (0%) | 6/8(++) (75%) | 6/8(++) | |
| Hyperaemic vessels | 0/10 (0%) | 6/8(++) (75%) | 6/8(++) | |
| Pyknotic nuclei | 0/10 (0%) | 2/8 (25%) | 2/8 | |
| Adrenals | Thickness external capsule | 2/10 (20%) | 3/7 (43%) | NA |
| Thymus | Hassal's bodies | 2/10 (20%) | 5/10 (50%) | 2/9 |
| Cortical invasivity | 2/10 (20%) | 2/10 (20%) | 8/9(++) | |
| Minerals | 2/10 (20%) | 5/10 (50%) | NA | |
| Stress | 0/10 (0%) | 5/10 (+) (50%) | NA | |
| Spleen | Lymphocyte hyperplasia | 4/10 (40%) | 2/10 (20%) | 5/10 |
| Infiltration in red pulp and periarteriolar zones (PALS) | 0/10 (0%) | 2/10 (20%) | 0/10 | |
| Brain | Eosinophilic cells in the cortex | 2/10 (20%) | 4/9 (44%) | NA |
| Eosinophilic cells in the thalamus | 3/10 (30%) | 4/9 (44%) | NA | |
| Condensed cells in the hypothalamus | 0/10 (0%) | 0/9 (0%) | NA |
NA: not analysed.