Table 1.
Optimization of the direct contact coculture model
| Attributes | Range | Optimized value |
|---|---|---|
| hCMEC/D3 seeding density | 50 000–250 000 cells/cm2 | 100 000 cells/cm2 |
| Astrocyte seeding density | 10 000–40 000 cells/cm2 | 40 000 cells/cm2 |
| Basement matrix | Collagen, poly-l-lysine, MaxGel, Fibronectin | Poly-l-lysine |
| Seeding time | 3, 5, 7, 9, 11, 13, 15, 17, 19 Days | 7 Days |
| Seeding order | Separate vs concurrent plating | Separate |
| Media | EBM-2 vs astrocyte medium | EBM-2 |
| Fetal bovine serum | Serum vs serum-free | Serum |
| Hydrocortisone | 100 nm–1.4 μm | 1.4 μm |
| HEPES | 10–50 mm | 25 mm |
| Lithium chloride | 0–10 mm | 0 mm |