Figure 4. Podocytes maturating on ECM produced by fibroblasts cultured in MMC conditions display enriched growth factor profile.

The densitometry analysis of multiplex growth factors membrane antibody array confirmed higher expression of many growth factors (Figure 4a, b; bFGF, TGF-α, TGF-β, TGF-β2, TGF-β3, IGF-I, IGF-II, AR, HBEGF, VEGF-A, SCF and PIGF), biding proteins (Figure 4c; IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4 and IGFBP-6) and receptors (Figure 4d; EGFR, IGF-I sR, MCSF-R, PDGFRα, PDGFRβ, SCFR, VEGFR2 and VEGFR3) in podocytes cultured (21 days) on decellularized matrix with MMC in comparison with other culture conditions (plastic, collagen coated, DCM without MMC).