Figure 6. Podocytes cultured on decellularized ECM produced by fibroblasts cultured in MMC conditions produced many extracellular matrix molecules.

Human podocytes were cultured in plastic or decellularized ECM rich well plates for up 21 days under differentiation condition (37 °C) (n=3). Podocytes cultured on decellularized matrix showed higher level of collagen type I (a), collagen type IV* (b), fibronectin (c) and laminin (d). Actin staining was performed to locate podocytes with EMC in the culture. *Minor cross reactivity of collagen type IV antibody was observed with collagen type I.