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. Author manuscript; available in PMC: 2021 Nov 25.
Published in final edited form as: Adv Funct Mater. 2020 Jun 8;30(48):2000545. doi: 10.1002/adfm.202000545

Figure 4: Co-culture of tumor organoids with fibroblasts potentiates pro-fibrotic changes in comparison to monoculture controls.

Figure 4:

A) Representative second harmonic generation (SHG) images of the Matrigel extracellular matrix, fibroblast mono-culture tissue, organoid mono-culture tissue and organoid/FB co-culture tissues. Scale bar, 40μm. B) Quantification of collagen area in each of the tissues measured at day 8 from the second harmonic generation images (Data are mean ± SD, * p < 0.05, N = 3 tissues, z-stack of 2 different areas of each tissue were imaged, one-way ANOVA followed by Tukey’s comparison test) C) Tissue elasticity as determined by atomic force microscopy (Data are mean ± SD, * p < 0.05, N = 3 tissues, minimum of 3 spots of each tissue, one-way ANOVA, Tukey’s comparison test was used).