Figure 1.

Co-culture model and real time MCF7 cells analysis. (A) Presentation of the 2D co-culture system and the protocol. (B) xCELLigence dynamic monitoring of MCF-7 cells, A representative graph from xCELLigence system: cell index (CI) profiles are the $\text{mean}\pm \text{SD}$ (duplicate) of each condition: Control (*, vehicle MCF-7 cells, alone), TCDD ($\$$, MCF-7 cells treated with $25\text{\hspace{0.17em}nM}$ TCDD), co-culture ($€$, MCF-7 co-cultured with hMADS) and coexposure (&, co-culture with TCDD). (C) The evolution of the CI for each condition was determined by analyzing the slope of the line in the interval (26–48 h). Each graph represents the mean slope (in bold) compared with the $\text{control}\pm \text{SEM}$ for six measurements. The numerical information $\text{mean}\pm \text{SEM}$ and $p$-values are provided in Table S1. (Kruskal–Wallis’s H test (nonparametric comparison of $k$ independent series) followed by a 1-factor ANOVA test (parametric comparison of k independent series), **$p<0.01$; *$p<0.05$). Note: ANOVA, analysis of variance; SEM, standard error of the mean; TCDD, 2,3,7,8-tetrachlorodibenzo-p-dioxin.