Figure 5.

Distributions of unbound dp10 molecules in a 5 μM solution of hNE incubated with dp10 at different molar ratios (as indicated on each graph, r represents the molar ratio of protein to dp10). The bottom trace represents a reference spectrum of dp10 in a protein-free solution. Only ions at +2 charge state are shown for clarity. Ions representing individual heparin oligomers are labeled according to Henriksen’s nomenclature,⁴⁷ where the three numbers in parentheses represent the oligosaccharide length, the total number of sulfate groups, and the total number of acetyl groups.