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. Author manuscript; available in PMC: 2021 Nov 25.
Published in final edited form as: Adv Funct Mater. 2020 Jun 3;30(48):2001232. doi: 10.1002/adfm.202001232

Figure 1: RNA sequencing of lymph node stromal and germinal center B cells shows niche associated heterogeneity of integrin-related genes.

Figure 1:

A) Schematic of lymph node structure comprised of distinct stromal cell populations inside and outside of B cell follicle. Non-endothelial stromal cells isolated from the peripheral lymph node are subjected to droplet-based single-cell RNA sequencing workflow. B) Single-cell RNA sequencing of nine uniquely identified clusters of peripheral lymph node non-endothelial stromal cells reveals the expression of mRNA encoding for proteins that bind to integrins. Violin plots represent the log-normalized gene expression (unique molecular identifier counts) for each cell grouped by the cluster. C) RNA sequencing comparison of in vivo integrin expression between naïve and germinal center B cells. D) RNA sequencing comparison of in vivo matrix metalloproteinase (MMP) expression between naïve and germinal center B cells. In C-D, data represent mean ± S.E.M. Significant differences determined using a 2-way ANOVA with Sidak’s multiple comparison test. ****p<0.0001; N=5-8.