Table 1. Demographics and disease history of patients from RDH and DD enrolled in this study.

Patient No.	Age	Indigenous	Clinical Diagnosis	Duration of Symptoms	Scabies Treatment	Course of Treatment	Collection Site	Microscopy Diagnosis	Sample Source
1	69	Yes	Ordinary Scabies*	3 months	Permethrin & Ivermectin	3 months	Right Upper Leg	Negative	DD
2****	4 moths	Not Stated	Ordinary Scabies	1 week	No	N.A.	Left Foot	Negative	RDH
			Ordinary Scabies	1 week		N.A.	Right Foot	Positive	RDH
3****	16	Yes	Ordinary Scabies	> 1 month	No	N.A.	Right Wrist	Negative	RDH
			Ordinary Scabies	> 1 month		N.A.	Left Axilla	Positive	RDH
4	2 months	Yes	Ordinary Scabies	2 weeks	Permethrin	4 days	Left Foot	Positive	RDH
5	78	No	Ordinary Scabies	2 months	No	N.A.	Right Palm	Negative	DD
6	11	No	Ordinary Scabies	4 weeks	No	N.A.	Right Thumb	Negative	DD
7	60	No	Ordinary Scabies	3 months	No	N.A.	Left Palm	Negative	DD
8	9	No	Ordinary Scabies	3 weeks	No	N.A.	Left Finger	Negative	DD
9****	2mos	Yes	Ordinary Scabies	> 2 weeks	Permethrin	> 2 Weeks	Abdomen	Negative	RDH
		Yes	Ordinary Scabies	> 2 weeks			Left Foot	Negative	RDH
10	55	Yes	Grade 1 Crusted Scabies**	Several months	Permethrin	2 weeks	Left Palm	Negative	DD
11	65	No	Grade 2 Crusted Scabies***	Several weeks	Benzyl Benzoate & Ivermectin	2 days	Right Elbow	Positive	RDH
12	91	Yes	Ordinary Scabies	3 months	No	N.A.	Right Arm	Negative	DD
13	68	No	Dermatitis	6 months	N.A.	N.A.	Left Palm	N.A.	DD
14	39	No	Dermatitis	3 months	N.A.	N.A.	Left Knee	N.A.	DD
15	78	No	Bowen’s Disease	6 months	N.A.	N.A.	Left Calf	N.A.	DD
16	1mo	No	Eczema	12 months	N.A.	N.A.	Right Palm	N.A.	DD
17	55	No	Seborrhoeic keratoses	Years	N.A.	N.A.	Right Arm	N.A.	DD
18	32	No	Tinea nigra	1 year	N.A.	N.A.	Right Palm	N.A.	DD
19	39	No	Seborrhoeic dermatitis	3 months	N.A.	N.A.	Midline Scalp	N.A.	DD
20	46	No	Dermatitis	12 months	N.A.	N.A.	Anterior Neck	N.A.	DD
21	38	Yes	Pityriasis versicolor	Several years	N.A.	N.A.	Right Flank	N.A.	DD
22	66	No	Psoriasis	NA	N.A.	N.A.	Dorsum of hand	N.A.	DD
23	55	No	Psoriasis	NA	N.A.	N.A.	Left Thigh	N.A.	DD
24	59	No	Psoriasis	NA	N.A.	N.A.	Left Leg	N.A.	DD
25	56	No	Psoriasis	NA	N.A.	N.A.	Right Elbow	N.A.	DD
26	84	No	Dermatitis	NA	N.A.	N.A.	Mid Back	N.A.	DD
27	Not Stated	No	Plaque Psoriasis	NA	N.A.	N.A.	Left Forearm	N.A.	DD
28	67	No	Psoriasis	NA	N.A.	N.A.	Right Knee	N.A.	DD
29	80	No	Psoriasis	NA	N.A.	N.A.	Right Arm	N.A.	DD
30	33	No	Eczema	NA	N.A.	N.A.	Left Elbow	N.A.	DD
31	29	No	Tinea	NA	N.A.	N.A.	Right Forearm	N.A.	DD

Patients were distributed in three groups: scabies group, which included patients clinically diagnosed with scabies who did not receive treatment at the time the samples were taken; scabies group treated, which included patients clinically diagnosed with scabies who received treatment before samples were taken; no scabies group, which included patients clinically diagnosed with non-specific conditions.

*Ordinary Scabies: infestation with Sarcoptes scabiei var. hominis, in the absence of features of crusted scabies (hyperkeratotic plaques or scaling with abundant mites) (Engelman et al., 2018)

**Grade 1 crusted scabies: infestation with Sarcoptes scabiei var. hominis with hyperkeratotic plaques or scaling with abundant mites, total score of 4–6

***Grade 2 crusted scabies: infestation with Sarcoptes scabiei var. hominis with hyperkeratotic plaques or scaling with abundant mites, with total score of 7–9 (Davis et al., 2013)

****Patient 2, 3 and 9 had an additional sample taken due to the presence of a second scabies lesion.

DD: Darwin Dermatology; RDH: Royal Darwin Hospital