Figure 7.

NFLP targets proline metabolism in breast cancer spheroids.A, abundance of proline M + 0 and M + 5 labeling in MCF10A hRAS^V12 spheroids upon ¹³C₅-glutamine incubation and treatment without (n = 3) or with NFLP (5 mm; n = 3). Analysis was performed at 5th day of treatment. B, relative abundance of intracellular proline levels in MCF10A hRAS^V12 spheroids upon treatment (5 days) without (n = 6), with THFA (n = 3) or with NFLP (n = 6). C, protein content in MCF10A hRAS^V12 spheroids treated for 5 days without (n = 6), with THFA (n = 3) or with NFLP (n = 6). Bar graphs show mean ± S.D. from biological independent samples and P-values were obtained with Mann-Whitney tests.