Table 2.

Kinetic parameters for PYCR1 inhibition by proline analogs^a

Analog	Km (μm)	kcat (s−1)	kcat/Km (m−1 s−1)	Ki (μm)
THFA	265 ± 17	69 ± 1	2.6 ± 0.5 × 105	2249 ± 121
l-Proline	374 ± 41	58 ± 3	1.5 ± 0.7 × 105	1718 ± 224
CPC	185 ± 9.0	35 ± 1	1.8 ± 1.1 × 105	1193 ± 61
l-T4C	269 ± 21	54 ± 2	2.0 ± 0.9 × 105	598 ± 39
l-T2C	232 ± 17	37 ± 1	1.5 ± 0.5 × 105	438 ± 29
NFLP	236 ± 21	52 ± 2	2.2 ± 0.9 × 105	99 ± 7.7

^aK_m and k_cat are the kinetic parameters for l-P5C determined at fixed NADH concentration of 175 μm. K_m, k_cat, and K_i were obtained using global fitting to the competitive inhibition model in Equation 1.