Table 1.

Comparison of binding and Gag-GFP release assays with yeast and HEK293 cells

	Yeast		HEK293
Gag-GFP release:
MA3*	Increased		Increased
Δp6	Efficient		Impaired
p6T*	Efficient		Impaired
p6A*	Efficient		Efficient (the combination of DNCA and p6A* abolished
ΔNCA	Impaired		Efficient the MA3* release-increasing effect)
	Bro1	Vps23	ALIX	TSG101
Binding to:
GST-MA	Yes	Yes	Yes	Yes (stronger binding to GST-p6)
MA-GFP	Yes	Yes	Yes
CA-GFP	Yes	Yes
Gag(MA3*/ΔNCA)-GFP compared with Gag-GFP	Reduced	Reduced
GST-p6	Yes	No	Yes	Yes
GagΔp6-GFP compared with Gag-GFP	Not reduced	Not reduced	Reduced	Reduced