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. 2021 Jan 13;295(52):17904–17921. doi: 10.1074/jbc.RA120.016105

Figure 2.

Figure 2

Purification of nsp1β and PCBP2 and their interaction with DNA and RNA.A, SDS-PAGE showing recombinant PRRSV SD01-08 nsp1β (∼23 kDa) and human PCBP2 (∼38 kDa). B, EMSA performed with a 20 μm concentration of a 34-nt ssDNA probe corresponding to a stretch of the nsp2-coding region of the PRRSV SD01-08 ssRNA genome where PRF occurs (Fig. 1B). Nsp1β and PCBP2 were combined independently or in tandem with the nucleic acid probe. The molar excess of each protein relative to the nucleic acid probe is shown below each lane. Lanes 9 and 10 contain a control DNA probe (CC2) (14) in which the C-rich region has been altered to adenine/guanine nucleobases. C, EMSA performed with a 20 μm concentration of a 34-nt ssRNA probe identical to a stretch of the nsp2-coding region of the PRRSV ssRNA genome where PRF occurs. Nsp1β and PCBP2 were combined independently or in tandem. The molar excess of each protein relative to the RNA probe is shown below each well.