Skip to main content
. 2021 Jan 13;295(52):17904–17921. doi: 10.1074/jbc.RA120.016105

Figure 5.

Figure 5

Analysis of innate immune suppression by expression of PRRSV nsp1β mutants. HEK293T cells were co-transfected with plasmids expressing innate immune response inducer MAVS, a firefly luciferase reporter gene under control of the IFN-β promoter, Renilla luciferase, and WT or mutant PRRSV nsp1β. Cells were lysed 18 h post-transfection, and the Renilla and firefly luciferase activities were measured. Firefly luciferase activity was normalized to Renilla activity in the same well. Three independent biological replicates with three technical replicates each are shown with S.D. values. Significance (p < 0.001) was assessed using an unpaired two-tailed Student's t test and is indicated with an asterisk.