Table 1.
Biophysical parameters of the PRRSV nsp1β:PCBP2:ssRNA 1:1:1 complex
Uncertainties are given in parentheses as 95% confidence intervals.
| Parameter | Experimental value | Experimental method | Dammin models |
|---|---|---|---|
| Sedimentation coefficient (S) | 2.84 (2.28–3.41) | SV | 4.22 ± 0.02a |
| Hydrodynamic radius (nm) | 6.11 (5.17–7.46) | SV | 4.40 ± 0.02a |
| Molecular mass Mb (kDa) | 68.8 (58.2–79.5) | SV | |
| Molecular mass M (kDa) | 86 ± 6 | SAXS, Primus | |
| Formula mass (Da) | 74,224.49 | ||
| Extrapolated scattering intensity at 0 angle I(0) | (0.089 ± 1.60)·104 | SAXS, Guinier | |
| Radius of gyration Rg (nm) | 3.900 ± 0.011 | SAXS, Guinier | |
| Extrapolated scattering intensity at 0 angle I(0) | (0.089 ± 1.35)·104 | SAXS, P(r) | |
| Radius of gyration Rg (nm) | 4.002 ± 0.008 | SAXS, P(r) | 4.044 ± 0.003 |
| Longest dimension Dmax (nm) | 13.5 | SAXS, P(r) | 13.98 ± 0.02 |
| Volume V (nm3) | SAXS | 165 ± 1 | |
| χ2 of fit | SAXS | ∼1.3 | |
| Normalized spatial discrepancy (NSD) | SAXS | 0.60 ± 0.01 | |
a
Calculated in Hydropro.
b
Using = 0.7166 cm³/g.