Figure 3.

Analysis of p53 dependence in the mechanism of action of the tenovins.A, Western blotting of p53 levels in ARN8 cells upon treatment with a 5 μm concentration of the indicated tenovins for either 2, 4, 6, or 8 h. B, SKNSH cells with normal WT p53 or stably expressing ddp53 were treated with a dose titration of the indicated tenovins for 72 h. Results are a single representative experiment with three technical replicates ± S.D. A total of three biological replicates were conducted. C, ARN8, HCT116 p53 WT, or p53 KO cells were treated with a fixed 5 μm dose of each tenovin and monitored for the confluence of the culture over 72 h by IncuCyte. Results are a single representative experiment with three technical replicates ± S.D. A total of three biological replicates were conducted. D, HCT116 p53 WT or p53 KO cells were treated with a 5 μm concentration of the indicated tenovins for 24 h prior to blotting for p53 and p53 targets. The total protein loading control for these blots is shown in Fig. S4.