Figure 4.

Phenotypic analysis of tenovins upon supplementation with nucleotides.A, p53 transcriptional activity assay (CPRG) in ARN8 cells treated with tenovins for 18 h supplemented with 100 μm uridine, 1 mm DHO, or 1 mm OA. Results are a single representative experiment with three technical replicates ± S.D. A total of three biological replicates were conducted. B, Western blotting of p53 and p53 targets in ARN8 cells treated for 24 h with a 5 μm concentration of the indicated tenovins supplemented with 100 μm uridine, 1 mm DHO, or 1 mm OA. The total protein loading for these blots is shown in Fig. S4. C, SRB assay in ARN8 cells treated with the indicated tenovins for 72 h supplemented with 100 μm uridine, 1 mm DHO, or 1 mm OA. Results are a single representative experiment with three technical replicates ± S.D. A total of three biological replicates were conducted.