Figure 6.

Inhibition of uridine uptake by tenovins.A, ARN8 or U2OS cells treated for 15 min with a 5 μm concentration of the indicated compounds, with uptake of ³H uridine measured by a scintillation counter. All data are normalized to protein levels prior to normalization to DMSO control (DMSO = 100). Error bars, S.D. of three technical replicates. B, Western blotting of U2OS cells treated for 24 h with a 5 μm concentration of the indicated compounds. C, U2OS cells treated for 24 h with the indicated compounds, with uptake of [³H]uridine measured by a scintillation counter. All data are normalized to protein levels for each individual compound as a proxy for cell number and then normalized to the DMSO control (DMSO = 100). Error bars, S.D. of three technical replicates.