Table 2.

Summary of tenovin-targeting profiles

Compound	Blockage of autophagic fluxa	p53 transcriptional activity induction	DHODH inhibition (enzyme assay)	DHODH inhibition (phenotypic identificationb)	Uridine uptake inhibition	SirT1 target engagement or biochemical inhibitionc
			μm
Tenovin 1	−	+	0.113	+	–	NT
Tenovin 6	+	+	0.5004	+	+	+
Tenovin 33	+	+	>20	+	+	+
Tenovin 39	+	+	>20	−	+	+
Tenovin 39OH	−	+	5.8	−	+	+
Tenovin 50	+	−	>20	−	+	+
Tenovin 50OH	−	−	>20	−	−	NT

^aBased on data presented in Ref. 12.

^bBased on recovery of p53 transcriptional activity induction and recovery upon the addition of uridine or orotate.

^cBased on cellular thermal shift data from Ref. 12 and data presented in Ref. 16. Tenovins 1 and 50OH were not tested (NT) in the target engagement study (CETSA) due to solubility issues at the concentrations required for this test (12).