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Bar diagram of full-length human Tau-2N4R protein with distribution of observed phosphorylation sites in Sf9 cells versus AD brain (>50 sites, mainly in C-terminal half, overlap 68%). A, 51 P-sites acquired by Tau in Sf9 cells (this study). B, 52 P-sites identified in AD-Tau; see collection of P-sites from AD brains (13). Red, Ser and Thr P-sites; purple underlined, SP or TP motifs; yellow highlight, high occupancy P-sites; red circles, P-sites important for AD diagnostics.
