Figure 2.

Lack of colocalization of nuclear murine GFP-Mx1 with nuclear PML bodies. Cultures of Huh7 cells in 35-mm plates were transiently transfected with expression vectors for murine GFP-Mx1 (A) or human GFP-MxA (B), fixed using 4% paraformaldehyde 2 days later, probed for PML using immunofluorescence methods, additionally stained with DAPI to visualize the nuclei, and imaged using three-color fluorescence. The figure illustrates representative cells. Pearson's correlation coefficients R (after Costes' automatic thresholding) in Aa, Ab, and B were 0.0, 0.25, and 0.0, respectively. Scale bars, = 10 μm.