Figure 3.

Disassembly of nuclear GFP-Mx1 bodies by 1,6-hexanediol. Huh7 and Mich-2H6 cells were transfected with either GFP-MxA vector (as a positive control) or GFP-Mx1 vector. 1–2 days later, the live cells were first imaged in PBS, and then the culture medium was changed to PBS supplemented with 5% (w/v) 1,6-hexanediol followed by imaging at different times thereafter (9, 33). A, positive control verifying the rapid disassembly of cytoplasmic GFP-MxA condensates (9, 33) in Mich-2H6 cells. B and C, a somewhat slower disassembly of GFP-Mx1 nuclear bodies after hexanediol exposure in both Mich-2H6 and Huh7 cells. Scale bar, 10 μm.