Figure 5.

Disassembly of murine GFP-Mx1 nuclear bodies in murine NIH 3T3 cells by 1,6-hexanediol and hypotonicity. NIH 3T3 cells in replicate 35-mm cultures were transiently transfected with the GFP-Mx1 expression vector. One day later, one culture was fixed with 4% paraformaldehyde, stained with DAPI, and imaged (A). Live-cell imaging was first carried out using the other cultures first in isotonic PBS, followed by the addition of 1,6-hexanediol in PBS (B), or switching to hypotonic ELB (C). B and C, representative live-cell images at the indicated times. 10 min after adding hypotonic ELB to the culture used in C, it was switched to isotonic PBS, and the same cell was imaged for the next 3 min (D). Scale bars, 10 μm.