Figure 1.

Experimental design and global overview of the PD-1–regulated phosphoproteome.A, experimental workflow. B, quantified total and differentially phosphorylated phosphosites. C and D, cluster analysis and principal component plots of normalized intensities for all phosphopeptides at 30 s (C) and 5 min (D) post-stimulation. E and F, volcano plots of differentially increased (red) or decreased (blue) phosphosites relative to the unstimulated control in response to αCD3 or αCD3+PD-L2 stimulation at 30 s (E) and 5 min (F). Horizontal dotted lines correspond to –Log10 adjusted p-value ≤ 0.05, and vertical lines correspond to FC ≥ ±1.3. G and H, number of shared and treatment-specific increased (red) or decreased (blue) phosphosites at 30 s (G) and 5 min (H).