Figure 4.

The NTS-DBL6ε reconstructed volume contains distinct regions and is consistent with the 2D classes.A, three orthogonal views of the 3D reconstructed NTS-DBL6ε molecule with head, tail, body, and feet, as indicated. Two pores P1 and P2 run through the protein. The arrows show the direction of rotation relative to the top left view. B, the top 15 2D classes, representing ≥90% of the particles used in the 3D reconstruction, paired with their corresponding back projection of the 3D model. All 3D volumes in this and subsequent figures were visualized using Chimera (74).