Figure 2.

Biophysical and NMR characterization of FOR005 V_L variants.A, Seeded ThT aggregation kinetics of patient protein FOR005, protein containing the single point mutation R49G and for protein coding for the germline sequence. In all cases, 5% seeds were added to the monomeric protein and incubated at 37 °C. B, Comparison of germline and patient fibrils both seeded with ex-vivo seeds. Superposition of the 2D NCACX correlation spectra for patient (black) and germline (red) fibrils. In both cases, ex-vivo seeds have been employed. For G102 and A13/A94, 1D traces were extracted along the ¹³C dimension (right). Fibrils formed by the germline protein show significantly reduced sensitivity and have increased line width (FWHM = 187 Hz for germline, FWHM = 155 Hz for patient fibrils).