Figure 8.

Catalytic domain structure comparison.A, visualization of the degree of preservation of GH43_sub24. The degree of conservation of amino acid residues in the catalytic domain of GH43_sub24 was visualized using the ConSurf server (RRID:SCR002320), the query for BLAST was set to Pc1,3Gal43A, and the conservation degree was analyzed based on 150 amino acid sequences in the ConSurf server (47,48, 49, 50,51). The conservation degree is shown in graded color. Preservation degrees are shown in a gradient with cyan for the lowest degree of preservation and blue for the highest. B, catalytic domain comparison of Pc1,3Gal43A and two GH43_sub24 galactanases. Shown are the catalytic centers of E208Q_Gal3 of Pc1,3Gal43A (white, PDB code 7BYV), BT3683 (cyan, PDB code 6EUI), and Ct1,3Gal43A (pink, PDB code 3VSF). Red, blue, and yellow, oxygen atoms, nitrogen atoms, and sulfur atoms, respectively. Residue names are shown for Pc1,3Gal43A/BT3683/Ct1,3Gal43A.