Table 2.

CLASS-D Phases and Aims

Phase #	Condition	Aims
Phase 0	Pre-sedation sleep study	Assess effects of CLASS on sleep (Aim 4)
Phase I	Light DEX titration	Induce EEG slow waves
Phase II	Light CLASS	Test effects of CLASS on behavior (Aim 2)
Phase III	Deep DEX titration	Induce loss of behavioral responsiveness
Phase IV	Deep CLASS	Test effects of CLASS on slow waves (Aims 1 and 5)
Phase V	Arousal	Test effects of CLASS on arousal thresholds (Aim 3)
Phase VI	Recovery	Drug elimination, transition to waking state
Phase VII	Post-sedation sleep study	Assess effects of CLASS on sleep (Aim 4)

Notes: Aims 1, 2 and 3 will be directly tested during the sedation protocol. Aim 4 will be completed using the sleep studies on nights preceding and following the sedation protocol. Aim 5 will be addressed by combining analysis of EEG data from the sedation protocol with a structural brain MRI acquired at a later date.