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. 2021 Mar 2;12(5):422–439. doi: 10.18632/oncotarget.27894

Figure 4. Effect of silencing combinations of 1B3 target gene expression on cell proliferation and apoptosis in selected human cancer cell lines.

Figure 4

Cells were transfected with 10-nM of either 1B3, ‘negative’ miRNA control (3A1), negative control siRNA (siPOOL), or a combination of siRNAs with a total RNAi concentration of 9–14 nM (see Table 3 for compositions). Non-transfected cells (mock) were also included to define baseline readout. (A) Nuclei count was determined after 96 h by staining with Hoeschst 33342 and imaging using the Thermo CellInsite Automated Imager. Values were normalized to mock transfected cells. (B) Caspase activation was measured 48 or 72 h after transfection using Caspase-Glo 3/7 assay (Promega). Values were normalized to mock transfected cells. The time point with the highest caspase activation is shown. All values are the mean of at least three independent replicates. The dashed line represents the mock value of 1.