Figure 2.

Mitochondrial Protein Changes during Astrocyte-to-Neuron Reprogramming

(A, B, E, and F) Micrographs showing immunostaining in astrocytes transduced with DsRed or Ascl1-ires-DsRed as indicated. Scale bars, 20 μm.

(C and G) Examples of scatterplots of the pixel intensity correlation between Tomm20 and Sfxn5 (C) or Prdx2 (G) in Ascl1-transduced cells on day 1 (left panel) and in reprogrammed cells on day 7 (right panel). Pearson’s coefficient as average of 3 cells/biological replicate; n = 3 biological replicates.

(D and H) Violin plots of the log₂ ratio of the intensity of the expression of Sfxn5 (D) or Prdx2 (H) normalized to Tomm20 intensity over time. Each dot represents 1 cell. 10 cells analyzed/biological replicate/condition/day. n = 3 biological replicates; ^∗∗∗p ≤ 0.001.