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. 2021 Mar 4;28(3):524–534.e7. doi: 10.1016/j.stem.2020.10.015

Figure 4.

Figure 4

Continuous Single-Cell Live Imaging Reveals Several Roles of Prdx2-Sod1 Activation in Neuronal Reprogramming

(A) Scheme of continuous live imaging and the analysis performed.

(B) Time course analysis of the percentage of cells acquiring neuronal morphology over double-transfected cells at the indicated time points. Data are shown as mean ± SEM. ∗p ≤ 0.05. n = 3 biological replicates for each group.

(C and D) Violin plot showing the lifespan of all cells analyzed irrespective of their final identity (C) and cells that died without converting (D), following expression of Ascl1-gRNA-GFP or Ascl1-gRNA-Prdx2-Sod1. n = 5 biological replicates for each group. ∗p ≤ 0.05

(E–G) Violin plots showing the lifespan (E and F) and speed of reprogramming (G) upon expression of Ascl1-gRNA-GFP or Ascl1-gRNA-Prdx2-Sod1. n = 5 biological replicates (color-coded) for each group. ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001.