Figure 1.
Derivation of Stem Cell Lines from Formative Epiblast
(A) Schematic of cell line derivation from E5.5 epiblast.
(B) Image of serially passaged E5.5-epiblast-derived culture. Scale bar, 100 μm.
(C) qRT-PCR analysis of marker gene expression relative to ESCs in 2iL ( =1) in AloX cells and EpiSCs maintained in either activin and FGF (AF) or activin, FGF, and XAV939 (AFX), normalized to beta-actin. Error bars are SD from technical triplicates.
(D) Immunofluorescent staining of EpiSCs and AloX cultures for early lineage markers. Scale bars, 150 μm.
(E) Immunostaining of embryoid body outgrowths for germ layer markers; DAPI in blue. Scale bars, 150 μm.
(F) Flow cytometry analysis of PGCLC induction at day 4.
(G) Immunostaining of day 4 PGCLC. Scale bars, 50 μm.