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. 2021 Mar 4;28(3):453–471.e8. doi: 10.1016/j.stem.2020.11.005

Figure 2.

Figure 2

Lineage Potency of FS Cells and Responsiveness to Differentiation Cues

(A) Neural differentiation assayed by quantification of Sox1::GFP-positive cells. Error bars represent SD from 4 independent experiments.

(B) Immunostaining of FS cells and EpiSCs during neural differentiation; DAPI in white. Scale bars, 100 μm.

(C) Lateral plate mesoderm differentiation and representative quantifications of the Flk1+Ecad− fractions by flow cytometry.

(D) Average efficiency of Flk1-positive cell production from FS cells and EpiSCs. n, independent cell lines assayed. Error bars represent the SD. ∗∗p < 0.01.

(E) Definitive endoderm differentiation protocol and representative quantifications of the Cxcr4+Ecad+ fraction.

(F) Average proportion of Cxcr4+Ecad+ double-positive cells from differentiation of FS and EpiSC lines. Error bars represent SD; ∗p < 0.05.

(G) T expression analyzed by qRT-PCR 6 h and 24 h after transfer into N2B27 medium with the indicated supplements; 2 μM XAV939, 20 ng/ml activin A, 10 ng/ml BMP2, 12.5 ng/ml Fgf2, and 3μM CH. Relative expression is normalized to GAPDH. Error bars are SD from two independent cell lines and two technical replicates.