Fig. 2.

Sensitivity comparison between LAMP-SNP assay and conventional PCR using the plasmid contained Pfkelch13^C580Y. (A) LAMP-SNP products were detected base on HNB color change, (B) Detection of LAMP-SNP products by agarose gel electrophoresis, (C) Amplified PCR products were visualized by agarose gel electrophoresis using F3 and B3 primer. In panels A, B and C: M, 100 bp DNA Ladder (Thermo Scientific); Lanes 1–9: 5×107 to 5×10−1 copies of 10-fold serial dilutions of purified plasmid DNA contained Pfkelch13^C580Y. Lane 10: No template control.