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. 2021 Mar 9;23(6):683–690. doi: 10.1016/j.jmoldx.2021.02.009

Figure 2.

Figure 2

Limit of detection (LoD) determination with extracted cultured severe acute respiratory syndrome coronavirus 2 RNA. Ct values of 10-fold and 2-fold serial dilutions of extracted cultured viral RNA obtained by microchip quantitative RT-PCR assay and measured on the AriaDNA analyzer with N1 (green dots) (A) and N2 (blue dots) (B) primers/probes. Ct values of 20 positive replicates per concentration of viral RNA (1 copy per well, 0.72 copies per well, and 0.25 copies per well) and 12 double distilled water negative controls per viral concentration, Ct values are determined as described in the Materials and Methods, and a Ct of zero indicates that the fluorescence signal did not cross the threshold limit within 45 cycles. n = 3 (A and B); n = 36 (C and D).