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. 2021 Mar 9;23(6):683–690. doi: 10.1016/j.jmoldx.2021.02.009

Figure 3.

Figure 3

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection of novel coronarvirus N1 (nCoVN1) primer/probe target (A) and novel coronavirus N2 (nCoV-N2) primer/probe target (B). The x axis shows input RNA copies per well, and the y axis shows positive results across all parallel reactions performed. Diamonds are experimental data points resulting from replicate testing at given concentrations (x axis) (20 replicate reactions per datum point for RNA concentrations up to 1.0 copies per well and 3 replicates for RNA concentrations >1.0 copies per well). The inner bold line represents a Probit curve, with outer dotted lines representing 95% CIs. C: Limit of detection (LOD) calculated from the Probit analysis.