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. 2021 Feb 4;16(3):446–457. doi: 10.1016/j.stemcr.2021.01.001

Figure 3.

Figure 3

Approaches for SN-subtype enrichment

(A) Schematics of proposed enrichment methods.

(B) Isolation of SN subtypes by immunopanning. Cells on day 25 were immunopanned using anti-TRKA, -TRKB, or -TRKC antibodies. Cells were cultured for 10 days (representative BF images, top) followed by IF for TRKA, TRKB, and TRKC (bottom).

(C) Timeline of culture conditions to promote mechanoreceptor enrichment (top). Representative BF images of each day (below).

(D) Flow-cytometry quantification of neurons expressing TRK receptors. The days when cells were replated and analyzed are indicated.

(E and F) SNs differentiated from SN-biased NCC replated on day 6 express mechanoreceptor markers TRKB, RET, BRN3A, TUJ1, VGLUT3, and PRPH by IF (E) and qRT-PCR (F).

(G) SN activity is modulated by hypo-osmotic medium. SNs were incubated for 1 min prior to data acquisition.

Data are normalized to untreated control (basal). In all experiments n > 4. Graphs show mean ± SD. ∗p < 0.05 using t test with Welch's correction. See also Figure S4.