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. 2021 Feb 23;13:629891. doi: 10.3389/fnagi.2021.629891

Figure 6.

Figure 6

Abnormal autophagic degradation in AD animal models and cell models. Immunofluorescence was used to observe the expression of the LC3 and Lamp1 proteins in the WT group, APP/PS1 group, and Aβ25–35 group mice; the red fluorescence shows the immunofluorescence staining of LC3 (A,C) or Lamp1 (B,D) protein. (E) Western blot showing the expression of the LC3 and Lamp1 proteins in the APPWT group and APPswe group cells, (F,G) and the relative gray value of the protein. (H) Western blot showing the protein expression of LC3 and Lamp1 in the control group and Aβ25–35 group cells. (I,J) The relative gray value of the protein. (K) Western blot showing LC3 protein expression in the control group and Aβ25–35 group cells before and after adding bafilomycin A1, and (L) the relative gray value of each group was compared. (M,N) PCR quantitatively analyzed the mRNA expression of Lamp1 in the APPswe group cells or Aβ25–35 group cells (*p < 0.05, **p < 0.01, ***p < 0.001; “ns” means there is no significant difference; n = 3).