Figure 7.

Effects of TFEB overexpression on autophagy in SH-SY5Y cells supplemented with Aβ_25–35. After overexpression of the TFEB protein in the control group or Aβ_25–35 group cocultured with BV2, (A) the expression of the TFEB, LC3, and Lamp1 proteins in the Aβ group, the Aβ + EV group, and the Aβ + TFEB overexpression adenovirus group was observed by Western blots, and (C–E) the relative gray values of each group were compared. (B) Western blotting was used to observe NLRP3, caspase-1, and IL-1β protein expression in BV2 cells, and (F–H) the relative gray values of each group were compared (*p < 0.05, ***p < 0.001; “ns” means there is no significant difference; n = 3).