FIGURE 2.

Immunomonitoring of CD4⁺ and CD8⁺ T cells in the peripheral blood. (A) CD4⁺ and CD8⁺ T cells (two‐tailed Mann‐Whitney test, p(CD4⁺ T cells) = 0.6274, p(CD8⁺ T cells) = 0.3274). (B) CTLA‐4⁺ CD4⁺ and CD8⁺ T cells (p(CTLA‐4⁺CD4⁺ T cells) = 0.0319 *, p(CTLA‐4⁺CD8⁺ T cells) = 0.0615). (C) PD‐1⁺ CD4⁺ and CD8⁺ T cells (p(PD‐1⁺CD4⁺ T cells) = 0.1550, p(PD‐1⁺CD8⁺ T cells) = 0.3431). (D) Proliferating (Ki‐67⁺) CD4⁺ and CD8⁺ T cells (p(Ki‐67⁺CD4⁺ T cells) = 0.0420 *, p(Ki‐67⁺CD8⁺ T cells) = 0.2065). (E) Survival analysis of patients depending on CD4⁺ and CTLA‐4⁺CD4⁺ T cells (Log‐rank (Mantel‐Cox) test, CD4⁺ T cells, p(nR >62.8% vs. R) = 0.0063 **, p(nR <62.8% vs. R) = 0.0251 *; CTLA‐4⁺CD4⁺ T cells, p(nR >13.9% vs. R) = 0.0025 **). Medians with interquartile range. CTLA‐4, cytotoxic T‐lymphocyte–associated protein 4; nR, non‐responder; PD‐1, programmed cell death protein 1; R, responder